Intravenous oxygen insufflation (IOI) changes the IL-1-Ra:IL-1β ratio in autologous conditioned serum

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Intravenous oxygen insufflation (IOI) changes the IL-1-Ra:IL-1β ratio in autologous conditioned serum

Background

Interleukin-1 (IL-1) is still regarded as the main offender that promotes the pro-inflammatory cascade in muscle injuries, tendopathies and especially in osteoarthritis. Thus, if present in high enough concentrations, IL-1receptor antagonist (IL-1Ra) has the potential to inhibit Interleukin-1. In this regard, autologous conditioned serum with an IL-1Ra/IL-1 ratio of at least >10 might fulfill optimal therapeutic effects. The purpose of this study was to analyze the combination of Regelsberger’s intravenous oxygen insufflation (IOI) with autologous conditioned serum (ACS) on IL-1Ra and IL-1 formation.

Methods

Venous blood from 15 patients which underwent intravenous oxygen insufflation (IOI) for routinely preventive purposes was taken before the first, the 6th, and the 9th session of intravenous oxygen insufflation. IL-1β and IL-1-RA levels were quantified from serum and from autologous conditioned serum (ACS).

Results

Previous intravenous oxygen insufflation treatments significantly reduced IL-1β levels in autologous conditioned serum from mean 67.85pg/ml (before the first treatment) down to mean 4.08pg/ml (before the 9th treatment). Post conditioning levels of IL-1Ra were not changed to any significant degree (before 1st/before6th/ before 9th treatment: 1038.37±140.51 / 900.30±79.24 /  902.84±95.68). Thus, the IL-1Ra:IL-1β ratio was altered on a molecule to molecule basis from a mean of 37.03:1 up to a mean 223.54:1 through the pretreatment.

Conclusion

IOI alters the IL-1Ra:IL-1ß ratio of autologous conditioned serum to a more favorable ratio which might mitigate the inflammatory cascade more efficaciously. Therefore, we suggest to perform intravenous oxygen insufflation on patients before they give blood for preparing ACS.

DOI: 10.21203/rs.3.rs-355919/v2
Interleukin-1, interleukin-1RA, intravenous oxygen, osteoarthritis, autologous conditioned serum,